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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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tumor necrosis factor alpha tnf α concentrations  (Proteintech)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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uk tnf α 60291 1 ig mouse  (Proteintech)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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tnf α elisa kits  (Proteintech)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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anti mouse tnf α antibody  (Bio X Cell)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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mouse anti tnf α monoclonal antibody  (Santa Cruz Biotechnology)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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mouse anti tnf α  (Proteintech)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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mouse tnf α elisa kit  (R&D Systems)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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tnf α e msel m0002  (Elabscience Biotechnology)
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by <t>enzyme‐linked</t> <t>immunosorbent</t> <t>assay</t> at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.
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Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by enzyme‐linked immunosorbent assay at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.

Journal: PCN Reports: Psychiatry and Clinical Neurosciences

Article Title: Acute trazodone treatment ameliorates behavioral abnormalities and improves subsequent short‐term memory in a lipopolysaccharide‐induced mouse model of acute brain dysfunction

doi: 10.1002/pcn5.70426

Figure Lengend Snippet: Plasma interleukin‐6 (IL‐6) concentrations at Days 1, 3, and 7. (a–c) Plasma IL‐6 concentrations at Days 1, 3, and 7, respectively. Plasma IL‐6 concentrations were measured by enzyme‐linked immunosorbent assay at Days 1, 3, and 7. Data are shown as box‐and‐whisker plots with individual data points ( n = 8 per group). Statistical analyses were performed separately at each time point using the Kruskal–Wallis test followed by the Steel–Dwass post hoc test. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; Sal, saline; TZD, trazodone.

Article Snippet: Plasma interleukin‐6 (IL‐6) and tumor necrosis factor‐alpha (TNF‐α) concentrations were measured using commercial ELISA kits according to the manufacturers' instructions (Mouse IL‐6 ELISA Kit, KE10007; Mouse TNF‐α ELISA Kit, KE10002; Proteintech Group, Inc.).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Whisker Assay, Saline